Cell bio lab report Essay Example for Free

Cell bio lab report EssayPurposeDuring this experiment we compared the hem agglutination reaction of control Con A solution at 2 mg/ml in Con A buffer with the hemagglutination reaction of your own purified Con A sample that you diluted previously at 2 mg/ml in Con A buffer. The purpose of this lab was to determine the strength of the reaction by performing serial dilutions on both the Con A sample and the control Con A sample, and determine through observations whether or not addition of galactose or mannose depart inhibit this reaction. I hypothesize that the Con A + galactose solutions allow for have partial agglutination and partial no agglutination, and the Con A + mannose solutions will have all no agglutination.Results wranglings A and B had half agglutination and half no agglutination, while row C had all no agglutination. Row D had half agglutination and half partial agglutination, while row E had 4 columns with agglutination and 8 columns with no agglutination. Row F had utter(a) agglutination throughout. Con A reaction plate (Row/Column)ReactionA1-A6 (Control)Agglutination/suppressA7-A12 (Control)No Agglutination/not containB1-B6 (Con A + galactose)Agglutination/inhibitedB7-B12 (Con A + galactose)No Agglutination/not inhibitedC1-C12 (Con A + mannose)No Agglutination/not inhibitedD1-D5 (Sample)Agglutination/inhibitedD6-D12 (Sample)Partial agglutination/inhibitedE1-E4 (Con A + galactose)Agglutination/inhibitedE5-E12 (Con A + galactose)No Agglutination/not inhibitedF1-F12 (Con A + mannose)No agglutination/not inhibitedG1-G12 ((-)Control)Partial agglutination/inhibitedH1-H12 (RBCs)Partial agglutination/inhibitedDiscussionMy hypothesis was proven correct, the Con A + galactose solutions did have partial agglutination and partial no agglutination, and the Con A + mannose solutions did have all no agglutination. This means that in the Con A + galactose solutions the ones that had agglutination the red cells aggregated with the lectin and sediment i n the form of a uniform layer that covered the whole bottom of the well including the slopes, and in the Con A + mannose solutions the ones that failed to have agglutination take lay out covered only part of the bottom. We found through this experiment that the control Con A sample and the Con A sample had very confusable strengths of reaction.ConclusionIn this experiment through serial dilutions on a 96 well plate we determined the strength of the each reaction on both the Con A sample and the control sample. We found through our experiment that the addition of galactose or mannose will partially inhibit or inhibit the reaction between our Con A sample and control Con A sample.